$B%7%g%&%8%g%&%P%($K$*$1$k(B
PARP$B$N5!G=2r@O(B
$BC4Ev6541!';0NX@5D>(B
$B3X@RHV9f!'#9#8#0#7#7#0(B
$B;aL>!'2,K\7<<#(B
Poly(ADP-ribose) polymerase-1(PARP-1)$B$O!"#2$D$N(Bzinc-finger$B$r$b$D%?%s%Q%/DNA$BB;=};~$K!"@ZCGC<$KFC0[E*$K7k9g(B
$B$7$F3h@-2=$5$l!"$5$^$6$^$J3KFb%?%s%Q%/ADP-ribose$B$rIU2C=E9g$9$k!#$3$N$3$H$h$j!"(BPARP$B$O=>Mh$+$i(BDNA$B=$I|$K4XM?$9$k$H9M$($i$l$F$-$?(B
$B$,!"(BDNA$B=$I|$K$*$1$k(BPARP$B$NLr3d$OL$$@ITL@$JE@$,B?$$!#$5(B
$B$i$K!"@5>o>uBV$K$*$1$k(BPARP$B$NLr3d$O!"$[$H$s$I8&5f$5$l$F$*$i$:!"(BPARP$B$N@8(B
$BJ*3XE*5!G=$OFf$N$^$^$G$"$k!#$=$3$G:#2s!"%7%g%&%8%g%&%P%(fuH/@8$X$N(B
PARP$B$N4XM?$r2r@O$7!"H/@8$H$$$&4pAC@8L?8=>]$K$*$1$k(B
PARP$B$NLr3d$N0lC<$r2rL@$7!"(BPARP$B$N@8J*3XE*5!G=$rC5$k;v$r;n$_(B
$B$?!#(B
1$B!!(B $B%7%g%&%8%g%&%P%(fuH/@8$O8E$/$+$iHs>o$K>\$7$/2r@O$5$l$F(B
$B$*$j!"3FH/@8CJ3,$K$*$1$kMM!9$J0dEA;R$N4XM?$,L@$i$+$K$J$C$F$$$k!#=>$C$F!"H/@8(B
$BCJ3,$K$*$1$k(BPARP$BH/8=$NJ,I[$d!"(BPARP$B2a>jH/8=$b$7$/$O0dEA;RGK2u$K$h(B
$B$kH/@8CJ3,$N0[>o$rD4$Y$k$3$H$G!"B>$N4{CN$N0dEA;R$H(BPARP$B$H$NAj8_:nMQ$rMF0W$K?dB,$G$-$k!#$=$3$G!"%7%g%&%8%g%&%P(B
$B%(fu$rH/@8CJ3,$4$H$K:NPARP$B93BN$rMQ$$$FLH1V@w?'$r9T$C$?!#$=$N7k2L!"H/@8=i4|$K$*$$(B
$B$F$OA4BN$,@w$^$C$F$$$?$N$KBP$7!"H/@88e4|$K$Ofu$NJ"ItCf?u?@7P@aIU6a$K6/$$@w?'(B
$B$,8+$i$l$?!#$3$N$3$H$+$i!"%7%g%&%8%g%&%P%($K$*$$$F!"(BPARP$B$O?@7PH/@8$K2?$i$+$N1F6A$r5Z$\$7$F$$$k2DG=@-$,$"$k$3$H(B
$B$,<(:6$5$l$?!#8=:_!"(BPARP$B0dEA;RGK2uBN$*$h$S0dEA;RF3F~BN$N:n@.!"$*$h$S$=$NI=8=7?$N2r@O$r?J$a(B
$B$F$$$k!#(B
2$B!!(B $BEv8&5f<<$N2a5n$N8&5f$+$i!"%7%g%&%8%g%&%P%($NH/@8CJ3,$K(B
$B$*$$$F(BPARP$B$O!"DcJ,;RNL(BGTPase RhoA$B$r2r$9$k%7%0%J%kEAC#7PO)$H$NAj(B
$B8_:nMQ$K$h$j!":YK&9|3J7A@.$dAH?%6K@-$K4X$o$k2DG=@-$,<($5$l$F$$$k!#99$KEv8&5f(B
$B<<$N$3$l$^$G$N8&5f$K$h$j!"(BPARP$B$,!"(BRho$B$N3h@-$r@)8f$9$k$H9M$($i$l$F$$(B
$B$k(BERM (Ezrin, Radixin, Moesin)$B%U%!%_%j!<%?%s%Q%/Merlin$B$HJ#9gBN$r7A@.$7!"(BMerlin$B$N3K0\9T!&3KGS=P$r@)8f$9$k2DG=(B
$B@-$,L@$i$+$K$J$j$D$D$"$k!#$D$^$j!"(BPARP-Merlin-RhoA$B$r2p$7$?3K(B$B!=(B$B:YK&Yeast Two-Hybrid$BK!$rMQ$$$F(BPARP$B$H(BMerlin$B$NAj8_:nMQ$r2r@O$7$?7k2L!"(BPARP$B$H(BMerlin$B$N7k9g$OD>@\E*$G$O$J$$2DG=@-$,<($5$l$?!#8=:_!"%7%g%&%8%g%&%P%($N(B
cDNA library$B$rMQ$$$F(BPARP$B$H(BMerlin$B$rCg2p$9$k%?%s%Q%/